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What are the origins of the field of Drosophila behavior?
As someone that has worked on fly behavior for many years, I’ve become more and more fascinated with the beginnings of the field. It...
Kaun Lab
Mar 29, 20244 min read
177 views


Does my mood affect my behavior experiments?
This is probably one of my most controversial opinions, but I think that the mood of a person can affect the outcome of an experiment. To...
Kaun Lab
Mar 28, 20243 min read
237 views


What ‘neutral’ odors can I use?
My lab performs lots of memory experiments: both Classical (Pavlovian) Conditioning experiments where a cue is presented at the same time...
Kaun Lab
Mar 28, 20244 min read
166 views


Should I food deprive (starve) flies for behavior?
As with all behavioral neuroscience animal model experiments, food deprivation is used as a way to increase motivational response. This...
Kaun Lab
Mar 27, 20244 min read
142 views


Should I use male or female flies for behavior?
Clearly "Should I use male or female flies for behavior?" is a trick question because there really is nothing limiting you from testing...
Kaun Lab
Mar 26, 20245 min read
170 views


How should I clean fly behavior equipment?
It’s important to clean fly behavior equipment because flies leave feces and cuticular hydrocarbons on the behavior arena surface, and...
Kaun Lab
Mar 25, 20243 min read
82 views


Do I need to perform a blinded experiment?
When you first learn experimental design, you learn all the best ways to control your experiments and prevent bias from infiltrating your...
Kaun Lab
Mar 24, 20244 min read
62 views

Should I watch the flies behave?
More and more the behavioral neuroscience field is moving toward using highly efficient and high-content behavioral tracking software to...
Kaun Lab
Mar 23, 20243 min read
79 views

What controls should I use for behavior experiments?
When I first started working on flies, I had no knowledge of genetics or insects. My limited undergraduate research experience included a...
Kaun Lab
Mar 22, 20245 min read
77 views


How do I perform optogenetic behavior experiments?
Optogenetics is a great way to temporarily control neuronal function. It’s typically used for light-controlled activation or inhibition...
Kaun Lab
Mar 21, 20244 min read
92 views


How do I humidify air and CO2?
Flies can sense humidity in their environment, and change their behavior when the air is too wet or dry (more on this on ‘Does humidity...
Kaun Lab
Mar 20, 20244 min read
103 views


How do I make and use a fly aspirator?
When you perform fly behavior experiments, you don’t want to use recently anesthetized flies since the common anesthesia that we use...
Kaun Lab
Mar 19, 20242 min read
148 views

Does the size of my flies matter for behavior?
The size of your flies could affect their behavior, but it may depend on the type of behavior you are measuring. Fly size can be directly...
Kaun Lab
Mar 18, 20243 min read
76 views


Do ambient odors affect fly behavior?
Drosophila have one of the best characterized olfactory systems of any animal (for a great overview, read Benton, 2022). Flies use...
Kaun Lab
Mar 17, 20242 min read
64 views

Does lighting affect fly behavior?
In order to get the most accurate behavior tracking, we create environments that are very artificial to flies. We also need to get very...
Kaun Lab
Mar 17, 20242 min read
43 views

How do I build a simple tracking system for fly behavior?
One of the nice things about working with flies is that you can build a simple, cost-efficient tracking system pretty easily. In the lab...
Kaun Lab
Mar 15, 20242 min read
104 views


How do I stop the flies from climbing?
Flies demonstrate negative geotaxis, an innate escape response during which flies ascend the walls of whatever chamber they are in. In...
Kaun Lab
Mar 15, 20241 min read
65 views

Does isolating flies affect behavior?
Flies have more complex social lives than have really been appreciated by a lot of the field of fly behavioral neurogenetics. I wrote a...
Kaun Lab
Mar 13, 20242 min read
65 views


Do thunderstorms affect fly behavior?
My PhD advisor, Dr. Marla Sokolowski, at the University of Toronto always told us that she felt Drosophila adult or larval behavior was...
Kaun Lab
Mar 12, 20241 min read
89 views

How long can I anesthetize my flies?
As you probably know, carbon dioxide (CO2) is the most popular anesthesia for fly pushing. It immobilizes flies quickly and flies wake up...
Kaun Lab
Mar 11, 20242 min read
265 views
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